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ICS-4 Institutsseminar: Prof. Vadim Cherezov (TSRI, La Jolla, USA)"Serial Femtosecond Crystallography of G Protein-Coupled Receptors”

Ort: Seminarraum des ICS-4, Geb. 15.1, E2, 1.OG Raum 249

18.02.2014 14:00 Uhr

Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA
Structural studies of G protein-coupled receptors (GPCRs), and other biomedically relevant membrane proteins and complexes, are hampered by challenges related to growing sufficiently large crystals capable of withstanding radiation damage and yielding high-resolution data at synchrotron sources. We have used an X-ray free electron laser (XFEL) with highly intense 50-fs pulses to minimize radiation damage and obtained a room temperature high-resolution GPCR structure using sub-10 mm crystals grown in a membrane mimetic gel-like matrix known as lipidic cubic phase. Microcrystals were delivered to the intersection point with an XFEL beam using a specially designed LCP injector, allowing to adjust LCP flowrate and minimize crystal consumption. Less than 0.3 mg of purified protein was used for a high quality dataset, making the LCP-SFX method attractive for structural studies of difficult-to-crystallize membrane proteins