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High-throughput screening of photosynthetic performance in the field using the Light induced fluorescence transient (LIFT) method

Beat Keller

Plants are exposed to large fluctuations in light and temperature diurnal and over the seasons. Photosynthesis is strongly linked to both of these abiotic factors. My interest is to quantify the photosynthetic response non-invasively and in a high time resolution. This will improve the understanding of the dynamic photosynthetic regulation and may lead to the identification of stress tolerant genotypes.

To measure the photosynthetic performance I use the light induced fluorescence transient (LIFT) method allowing high-throughput plant phenotyping (Soliense Inc.). The integrated fast repetition rate (FRR) technology allows induction of chlorophyll fluorescence from approximately 1m distance within milliseconds from any screening platform. The fluorescence response is linked to several photosynthetic traits, e.g. efficiency of charge separation within the reaction centers or electron transport time constants. Depending on the research question I am measuring in the lab, greenhouse with automated platform or in the field.

References

Osmond, B., Chow, W.S., Wyber, R., Zavafer, A., Keller, B., Pogson, B.J., and Robinson, S.A. (2017). Relative functional and optical absorption cross sections of PSII and other photosynthetic   parameters monitored in situ, at a distance with a time resolution of a few seconds, using a prototype Light Induced Fluorescence Transient (LIFT) device. Funct. Plant Biol. (in press).

Keller, B., Manzanares, C., Jara, C., Lobaton, J., Studer, B., and Raatz, B. (2015). Fine-mapping of a major QTL controlling angular leaf spot resistance in common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 1–14.

Walter, A., Grieder, C., Last, L., Keller, B., Hund, A., and Studer, B. (2014). The Swiss plant breeding sector – a spatial, temporal and thematic analysis. Agrar. Schweiz 5, 366–373.


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