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Nuclear magnetic resonance (NMR) and Magnetic Resonance Imaging (MRI)

Systems for non-invasive measurements of plant structure and function

Our MRI installations are a non-invasive modality allowing to measure dynamic changes in structural and functional traits of plant and soils under realistic environmental conditions.

Below ground applications

MRI enables imaging of roots growing in soil and thus makes it possible to analyse whole root architecture in three dimensions (Fig 1). The method yields detailed information on root traits such as mass, length, diameter, tip number, branching angles, spatial distribution and growth rates (van Dusschoten et al., 2016). For belowground storage organs like sugar beet, additional traits such as internal structure or shape development can be measured (Metzner et al., 2014).
To fit the root imaging setup, plants are typically grown in tubes of 8.1 cm inner diameter and 20-40 cm height, while the plant shoot may be up to 100 cm in height. The tubes are handled by a robotic system, enabling fully automated long-term measurement series of about 20 plants per day. Depending on the number of measurements per plant, we can run about 20-40 experiments per year. Examples of plant species of which root traits or bulky organs can be measured are maize, bean, barley, wheat, sugar beet or potato. The root structure of plants with very thin roots such as Arabidopsis cannot been measured.
The analysis of data gathered with MRI for one plant per pot is semi-automated by a software tool called NMRooting (van Dusschoten et al., 2016). Further, there is high flexibility to combine MRI with other methods (e.g. gas exchange or non-invasive monitoring of plant water distribution (Sydoruk et al. 2016).
Several scientific projects have been executed using the above MRI approach on roots. Soil density, for example, influences root length, diameter and tortuosity. Soil moisture depletion was observed to shift root growth to the wetter and deeper parts of the soil. Further, combining root imaging with soil water content imaging (with MRI) and a split root system enabled the differentiation between seminal and nodal root water uptake.

Root_growthFigure 1 Root growth of a maize plant in soil monitored 6, 9, 12, and 15 days after sowing displayed with MRI (2D maximum intensity projection of MRI data, adapted from van Dusschoten et al. 2016)

Above ground applications

For above ground studies, typical applications of high-field MRI on plants are the quantitative and non-invasive micro imaging of parameters such as water content, anatomy and sap flow. The most typical subjects are herbaceous plants of modest size, but if needed large trees can be handled as well. Our largest imager is able to accept potted trees of up to a height of 4.5 meters, and is able to measure xylem and phloem sap flow in stems of up to 8 centimetres in diameter.

One of the most unique features of MRI as applied on plants is that it allows the direct imaging of xylem and phloem sap flow inside the living plant. Recent applications of the MRI micro imaging and MRI flow imaging in our lab include imaging of xylem cavitation in grape stems and petioles (Hochberg et al 2016), of the effect of girdling on the xylem integrity of the tomato peduncle (Van der Wal et al, 2017), or the dynamics of phloem sap flow in gymnosperm trees (Liesche et al, 2015). An example of MRI micro imaging is a study on oak, in which quantitative micro imaging was successfully used to couple stem diameter changes, as measured by conventional point dendrometers, to changes in stem water content.

Mobile NMR sensors

NMR and MRI are unique in that they make it possible to quantitatively and non-invasively measure the presence and movement of water. Unfortunately, conventional equipment for NMR and MRI is usually large, expensive, and only suitable for use in a laboratory environment. Plants then need to be brought to the scanner, rather than the other way round.
In order to bring NMR technology to the field, we developed novel, small scale portable NMR devices that can be used in a sensor-like fashion (Windt and Blümler, 2015). In its simplest form, such NMR sensors consist of scaled down permanent magnets. They can be used, almost like a dendrometer, to measure dynamic changes in the absolute water content of fruit, leaves or stems (Lechthaler et al, 2016). By employing basic NMR relaxometry, the information that can be gained from these small scale NMR sensors can be extended, allowing the instantaneous, non-invasive measurement of changes in dry matter content as well. In this way, the development of yield (dry matter deposition) in terminal organs such as bean pods can be monitored, over a period of weeks if desired, without harvesting the plant (Fig. 2).
In addition to this, we are working to establish small scale, mobile NMR imagers. Our imagers are based on the same mobile permanent magnets that are used for the NMR sensors, but are fitted with imaging gradients and mobile, battery powered amplifiers to control them. Mobile imaging of plants is still in its infancy, but prototypes already have shown to be capable of low resolution MRI, as well as 1D xylem sap flow velocimetry (Windt and Blümler, 2015).

Figure 2 Bean pod in mobile NMR sensorFigure 2 Bean pod in mobile NMR sensor


Selected Publications

Metzner, R., van Dusschoten, D., Bühler, J., Schurr, U., Jahnke, S., 2014 Belowground plant development measured with magnetic resonance imaging (MRI): exploiting the potential for non-invasive trait quantification using sugar beet as a proxy. Frontiers in Plant Science

Liesche J, Windt C, Bohr T, Schulz A, Jensen KH (2015) Slower phloem transport in gymnosperm trees can be attributed to higher sieve element resistance. Tree Physiology 35: 376-386.

Windt CW and Blümler P (2015) A portable NMR sensor to measure dynamic changes in the amount of water in living stems or fruit and its potential to measure sap flow. Tree Physiology 35: 366-375.

Sydoruk, V.A., Fiorani, F., Jahnke, S., Krause, H.J., 2016 Design and Characterization of Microwave Cavity Resonators for Noninvasive Monitoring of Plant Water Distribution. IEEE Transactions on Microwave Theory and Techniques 64, 2894-2904.

Hochberg, U., Albuquerque, C., Rachmilevitch, S., Cochard, H., David-Schwartz, R., Brodersen, C.R., McElrone, A., Windt, C.W., 2016 Grapevine petioles are more sensitive to drought induced embolism than stems: evidence from in vivo MRI and microcomputed tomography observations of hydraulic vulnerability segmentation. Plant, Cell & Environment 39, 1886-1894.

Lechthaler S., Robert EMR, Tonné N, Prusova A, Gerkema E, Van As H, Koedam N, Windt CW (2016) Rhizophoraceae mangrove saplings use hypocotyl and leaf water storage capacity to cope with soil water salinity changes. Frontiers in Plant Science 7.

van Dusschoten, D., Metzner, R., Kochs, J., Postma, J.A., Pflugfelder, D., Buehler, J., Schurr, U., Jahnke, S., 2016 Quantitative 3D Analysis of Plant Roots growing in Soil using Magnetic Resonance Imaging. Plant Physiology.

van de Wal BAE, Windt CW, Leroux O, Steppe K (2017) Heat girdling does not affect xylem integrity: an in vivo magnetic resonance imaging study in the tomato peduncle. New Phytologist: in press


The 4.7 Tesla system

MRI_47T_jpg

Wide-bore, 310 mm magnet, equipped with a 300 mT/m 210 mm wide gradient system. Different r.f. coils available.

  • 63 mm quad birdcage
  • 104 mm quad birdcage
  • 170 mm quad birdcage
  • 40 mm openable birdcage
  • small r.f. coils manufactured locally
  • Heavy objects up to 500 kg can be lifted into the magnet using a crane. Objects up to 200 kg can be positioned with a computer-controlled positioning system for repetitive studies with an accuracy of about 20 µm.
  • environmental control systems complete the system.

 1.5 T MRI am IBG-2: Pflanzenwissenschaften

The 1.5 Tesla system

The split coil magnet is unique in its kind. It is equiped with 50 mT/m 380 mm and 800 mT/m 120 mm planar gradient sets. Different r.f. coils are available

  • 63 mm solenoid
  • 140 mm solenoid
  • several home-build splittable/wrappable solenoids
  • Heavy objects, like trees with large pots/containers can be lowered into a pit a 2 meters deep. Objects up to 5 meter can be measured.

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