Methods and Technologies at the
Jülich Plant Phenotyping Centre
Plant Growth (Leaf Area) and Fluorescence
Imaging Pulse Amplitude Modulated Fluorometry (PAM) device moved by a xy-positioning system across a population of plants in pots, trays or petri dishes. The blue light results from the fluorimeters’ saturation pulse.
Determination of chlorophyll-fluorescence images by PAM light protocoll.
- segmentation of fluorescent areas
- analysis of projected area and chlorophyll-fluorescence parameters
Example of results
- growth analysis based on fluorescent pixels
- chlorophyll-fluorescence analysis
The graph shows colour-coded images of plants under normal conditions (left), drought (centre) and cold stress (right). Light blue to green colouration indicates low, dark-blue to violet indicates high values.
Jansen, M.; Gilmer, F.; Biskup, B.; Nagel, K. A.; Rascher, U.; Fischbach, A.; Briem, S.; Dreissen, G.; Tittmann, S.; Braun, S.; De Jaeger, I.*; Metzlaff, M.*; Schurr, U.; Scharr, H.; Walter, A.
Simultaneous phenotyping of leaf growth and chlorophyll fluorescence via GROWSCREEN FLUORO allows detection of stress tolerance in Arabidopsis thaliana and other rosette plants, Functional Plant Biology, 36 (2009) 11, 902 - 914