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Methods and Technologies at the
Jülich Plant Phenotyping Centre

GROWSCREEN fluoro

Plant Growth (Leaf Area) and Fluorescence

growscreen fluoro

Data Acquisition

Imaging Pulse Amplitude Modulated Fluorometry (PAM) device moved by a xy-positioning system across a population of plants in pots, trays or petri dishes. The blue light results from the fluorimeters’ saturation pulse.

Determination of chlorophyll-fluorescence images by PAM light protocoll.

Image Analysis

  • segmentation of fluorescent areas
  • analysis of projected area and chlorophyll-fluorescence parameters

Example of results

  • growth analysis based on fluorescent pixels
  • chlorophyll-fluorescence analysis

FV/Fm-values

GROWSCREEN fluoro

The graph shows colour-coded images of plants under normal conditions (left), drought (centre) and cold stress (right). Light blue to green colouration indicates low, dark-blue to violet indicates high values.

GROWSCREEN fluoro - Trockenstress und Kälte

Literature


Jansen, M.; Gilmer, F.; Biskup, B.; Nagel, K. A.; Rascher, U.; Fischbach, A.; Briem, S.; Dreissen, G.; Tittmann, S.; Braun, S.; De Jaeger, I.*; Metzlaff, M.*; Schurr, U.; Scharr, H.; Walter, A.

Simultaneous phenotyping of leaf growth and chlorophyll fluorescence via GROWSCREEN FLUORO allows detection of stress tolerance in Arabidopsis thaliana and other rosette plants, Functional Plant Biology, 36 (2009) 11, 902 - 914

Additional Information

 Jülich Plant Phenotyping Centre

 

jppc_200x_jpg

 

a development of

Growth and Metabolism and

ET (Enabling Technology)

                                            

link to the research area

JPPC

 

Contact

Dr. Fabio Fiorani

 


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