Our group works in the field of Alzheimer’s disease research. We are interested in the mechanisms, which turn native, well behaving cellular peptides or proteins into toxic aggregates.
At the HHUD we host an analytical ultracentrifuge (AUC) which is equipped with absorption optics. A four hole rotor allows to make measurements in three standard double sector sample cells with 1 cm optical path length. Temperatures can be chosen between 4 and 30°C. With this instrument sedimentation velocity as well as sedimentation equilibrium centrifugation experiments for the determination of size and shape of macromoelcules or macromolecular assemblies, native molecular weights, and equilibrium constants can be performed under a wide varity of solution conditions. AUC is a first principle method.
Also two preparative ultracentrifuges are on place, one table-top machine with a rather small rotor allowing centrifugation in 1 ml lab tubes, the other one equipped with rotors housing tubes of 10 to 30 ml volume. (up to 320,000 x g)
Either fluorescence correlation spectroscopy (FCS) or fluorescence intensity distribution analysis (FIDA) can be performed with an Evotec Insight instrument, which is equipped with an He-Ne-laser emitting at 543.5 nm.
Besides several instruments for UV/vis and fluorescence spectroscopy the institute offers a stopped- flow-instrument (Bio-Logic SAS, Claix, France) in combination with fluorescence detection for the measurements of rate constants.
A flow field flow fractionation instrument AF4 (Eclipse, Wyatt, Dernbach, Germany) equipped with triple detection systems further extends our instrumental equipment. Compared to size exclusion chromatography AF4 allows the separation of macromolecules or macromolecular assemblies according to their hydrodynamic properties without the presence of a solid phase and therefore eliminates problems arising from unwanted interactions between analyte and solid phase.
List of Collaborations
- PD Dr. Bernd König, Institute for Complex Systems-6, Structural Biology, RC Jülich
- Prof. Dr. Reza Ahmadian, Institut für Biochemie und Molekularbiologie II, Universitätsklinikum Düsseldorf
- Dr. Shai Rahimipour, Drug Discovery, Design and Delivery Research for Neurodegenerative Diseases, Bar Ilan University, Ramat Gan, Israel
- Prof. Dr. Jens Pahnke, Neurodegeneration Research Lab, Deutsches Zentrum für Neurodegenerative Erkrankungen e.V., Magdeburg
- Dr. Ingrid Vetter, Max-Planck-Institut für molekulare Physiologie, Abt. Kristallographie, Dortmund
- Prof. Dr. Wolfgang Linke, Dept. of Cardiovascular Physiology; Ruhr University Bochum