Research Project SynBio
The Helmholtz Initiative on Synthetic Biology will focus novel synthetic tools for analysis, design and control in health, disease and biotechnology. The project within this frame will focus on the developments of optogenetic tools for the manipulation of cellular processes
Abstract: we will establish cortical cultures and retinal tissue explants as well-defined model systems to a) functionally validate optogenetic tools, and to b) study and control signaling in these model systems. Using adeno-associated viruses as gene ferries, we plan to express ChR-2 as well as optogenetic sensors in a) cortical neurons in cortico-striatal co-cultures, b) in bipolar cells of the intact retina and c) in selected tumor and immune cells. Responses are recorded using fluorescent optogenetic sensors as well as multi-electrode arrays or classical electrophysiology. Coupling these optogenetic tools to genetic readout systems will enable time- and space-confined control over therapeutic transgene expression in various cell types, tissues, and organisms.
Principal Investigator(s): Baumann, Arnd; Müller, Frank; Offenhäusser, Andreas
Partner: SynBio federates 12 partners: German Cancer Research Center (DKFZ), Research Center Jülich (FZJ), Karlsruhe Institute of Technology (KIT), Helmholtz Zentrum München (HMGU), Helmholtz Center for Infection Research (HZI), as well as the external partners Heidelberg University (Uni HD), Freiburg University (Uni FR), ETH Zurich - Department of Biosystems Science and Engineering (D-BSSE)
Webpage: ----- (under construction)
Project start: 01.09.2012
Probable end: 31.12.2014