Before a novel biolectronic devices as for instance a bioelectrochemical rectifier can be realized on micro or nanometer scale, it is recommendable to first evaluate immobilization strategies and to characterize the electrochemical properties of the identified (redox active) biomolecules at macroscopic electrodes. A variety of immobilization strategies exist enabling the wiring of biomolecules to conducting substrates. We primarily focus on the development and investigation of new immobilization strategies to optimize the electrical communication between electrode and biomolecule. Besides covalent binding strategies we engineer recombinant proteins and incorporate specific binding tags (e.g. his-tag) into the amino acid sequence to enable a high affinity immobilization. The immobilization process and the charge transfer kinetics of the biomolecules are characterized by electrochemical, spectroscopic, and scanning probe techniques.
The 2D macroelectrodes are furthermore used to evaluate the functionality of novel bioelectronic devices where redoxactive biomolecules are utilized as building blocks. Here, we are using on the one hand biological recognition for sensing applications and on the other hand bioelectronic properties like current rectification to realize logical operations. The findings gained from investigations on 2D macroelectrodes can later be used for the assembling of micro- and nanosized bioelectronic junctions.