Metallomics integrates work on metalloproteins, metallo-enzymes and other metal-containing biomolecules.
Combination of elemental and biomolecular mass spectrometry
Elemental and biomolecular mass spectrometric methods are highly complementary: elemental mass spectrometry methods, such as ICP-MS and LA-ICP-MS offer very sensitive element analysis in the trace and ultra-trace concentration range with multielement capability and the excellent and uniform sensitivity. In contrast, molecular weight determination and structural information is completely lost during the ionization step so that these features have to be provided by biomolecular mass spectrometry and in particular by ESI- and MALDI-MS.
Imaging LA-ICP-MS combined with proteome studies on brain tissue (starting from cryocutting an entire brain, scanning entire brain slices by LA-ICP-MS). Structures of interest are identified and the proteome is extracted from corresponding blocks of tissue. 2D electrophoresis gels are further analyzed by imaging LA-ICP-MS to identify metal-containing spots. From analogous gels spots are picked, tryptically digested, and the corresponding proteins identified by MALDI- or ESI-MS techniques.
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Becker J.S., et al. Metallomics 2 (2010) 104-111.
Lobinski, R. Becker, J.S., Haraguchi, H., Sarkar, B. Metals in biological systems and -omics: guidelines for terminology and critical evaluation of analytical chemistry approaches (IUPAC Technical Report), International Union of Pure and Applied Chemistry, 82 (2010) 493-504.
Becker, J.S. (ed.) Special issue “Bioinorganic Mass Spectrometry and Metallomics”, Int. J. Mass Spectrom. 307 (1-3) 1-252.