Nano-LA-ICP-MS using near field effect for bioimaging of metals at nanometre scale
The basic idea of further improving the spatial resolution of laser ablation in the nanometre (nm) scale (to 50 nm) was the insertion of a thin Ag (or Au) needle into a defocused laser beam using the near-field effect in laser ablation. The tip of the thin needle acts like a "nanomagnifier" (like the "antenna effect"-a similar principle to the "lightning conductor" on a house). Photons are focused on the tip of the needle due to the near-field effect, the focusing is 300 × better than the best focusing lens and a strong field enhancement was observed.
Becker, J.S., Kayser, M., Gorbunoff, A. et al. German Patent, 2008.
Zoriy, M., Becker, J.S. Rapid Commun. Mass Spectrom. 23 (2009) 23-30.
Zoriy, M., Mayer, D., Becker, J.S. J. Am. Soc. Mass Spectrom. 20 (2009) 883-890.
Zoriy, M., Kayser, M., Becker, J.S. Int. J. Mass Spectrom. 273 (2008) 151-155.
Becker, J.S. Int. J. Mass Spectrom. 289 (2010) 65-75.
Development of Lasermicrodissection-ICP-MS (LMD-ICP-MS)
Another possibility is the laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) as novel mass spectrometric imaging technique, which is intended to investigate elemental distribution in small areas of biological tissue with high spatial resolution down to the low-micrometre range and below. The LMD-ICP-MS technique combines a laser microdissection apparatus (LMD) with sensitive inductively coupled plasma mass spectrometry (ICP-MS) to utilize the tightly focused laser beam in LMD for the ablation of small size tissue.
Becker, J.S., Niehren, S., Matusch, A., Wu, B., Hsieh, H.-J., Kumtabtim, U., Hamester, M., Plaschke-Schlütter, A., Salber, D. Int. J. Mass Spectrom. 294 (2010) 1-6.
Wu, B., Niehren, S., Becker, J.S., J. Anal. At. Spectrom., 26 (2011) 1653-1659.