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Seminar by Dr. Francesco S. Pavone

European Laboratory for Non Linear Spectroscopy, University of Florence (Italy)

09 Oct 2015 14:00
09 Oct 2015 15:00
Lecture room 2009, Jülich GRS building (16.15)

Modern optics and spectroscopy are offering promising non-invasive solutions to potentially improve diagnostic capability on tissues, as demonstrated by the extensive use of non-linear laser scanning microscopy for tissue imaging in the past decade.

The recent development and integration of multiple non-linear microscopy techniques in a single instrument has provided new opportunities for integrating morphological and functional information and for correlating the observed molecular and cellular changes with disease behaviour. In particular, multimodal non-linear/linear imaging is able to perform a morpho-chemical quantitative analysis in tumour cells and tissue specimens, providing a high-resolution label-free alternative to both histological and immunehistochemical examination of tissues. Although up to now limited to optical research labs, multimodal non-linear imaging is becoming increasingly popular among medical doctors and has the potential to find a stable place in a clinical setting in the near future.

In this talk, a brief overview on the non linear and linear laser brain imaging techniques will be displayed both for label free and specific labeling detection.

Morpho-functional characterization of tissue will be displayed as an interesting tool for early diagnosis of pathologies: different kind of approaches will be shown for in vivo imaging assisted surgery operation or as tools to support anatomo-pathologists decision.

For example, several techniques, useful to create a new 3D histological analysis will be shown, and a fiber based endoscope based on multidimensional spectral detection (one photon fluorescence, lifetime and Raman detection) will be described with particular applications to tumor detection.