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Institute for Advanced Simulation (IAS)

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Seminar by Dr. Antonio Sechi

Institute of Biomedical Engineering, Uniklinik RWTH Aachen (Germany)

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28 Apr 2015 14:00
end
28 Apr 2015 15:00
venue
Lecture room 2009, Jülich GRS building (16.15)

Gas2L1 (also known as GAR22beta) is a poorly characterised protein that interacts with both actin microfilaments and microtubules. We found that Gas2L1 is highly expressed in mouse brain (cortex and hippocampus), thyroid and testes. Gas2L1 KO male mice have reduced fertility, lower actin levels in testes and impaired motility and disorganised ultrastructure of spermatozoa. In cells, we found that absence of Gas2L1 expression results in increased cell motility and focal adhesion (FA) turnover.

Accordingly, either its over-expression in normal cells or re-expression in Gas2L1 KO cells reduces cell motility and FA turnover. Using fluorescence recovery after photobleaching, we found that the interaction of Gas2L1 with actin filaments is stronger than that with microtubules. Moreover, we determined that Gas2L1 interacts with end-binding protein 1 (EB1) via a novel non-canonical amino acid sequence and that Gas2L1-EB1 interaction is required for the ability of Gas2L1 to modulate cell motility.

Collectively, these findings support an essential role of Gas2L1 in the regulation of cytoskeleton-dependent biological processes.


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