Crosslinking mass spectrometry has become a powerful technique for studying protein-protein interactions. Crosslinking for protein-protein interaction studies enables visualization of interacting regions by allowing researchers to create distance maps within protein complexes or individual proteins. XL-MS can be used in parallel with high-resolution techniques such as cryo-electron microscopy (cryo-EM) or X-ray crystallography to obtain structural information. Crosslinking experiments reveal distance constraints between regions within the protein, allowing the generation of low-resolution three-dimensional structures or a general topology of the protein structure. For protein complexes, XL-MS can also be used to determine protein complex organization.
Contact: Dr. Beatrix Santiago-Schübel