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LIFT - Light induced fluorescence transient method

The LIFT provides the opportunity to quantify photosynthetic processes from a distance and can be used as a high –throughput system scanning over crop canopies.

The light induced fluorescence transient (LIFT) method uses a series of excitation flashlets to induce variable fluorescence (Fv) and to monitor fluorescence relaxation. The resulting fluorescence transient reflects the coupled kinetics of QA reduction and its subsequent reoxidation. For the reduction phase, normalizing Fv with a maximum fluorescence level ( Fm) provides information about the quantum efficiency of the photosystem II (Fv/Fm in the dark and Fq’/Fm’ in the light) reflecting the amount of photosynthetically transported electrons per photon the most commonly measured photosynthesis fluorescence parameter.
Furthermore from the re-oxidation phase of the transient kinetics of the electron transport can be quantified and from the initial increase in fluorescence the size of the functional and optical absorption cross section of photosystem II. In addition the LIFT device is equipped with a spectrometer in the 326-812nm spectral range and two RGB cameras.
The development of the LIFT has been in close interaction with researcher of Forschungszentrum Juelich and the producer Zbigniew Kolber (www.soliense.com). The previous model of the current LIFT was a stationary large LIFT setup equipped with a laser operating at a distance of about 20m. The reduction in size and eye safe LED operation allowed the new LIFT system to be integrated in various positioning systems for high throughput phenotyping while remaining its high resolution for quantifying photosynthetic fluorescence parameters.
In Forschungszentrum Juelich 4 LIFT system are available. These are used from lab to field conditions. In the field the LIFT is integrated on the manually moved field4cycle, the self-driving fieldcop and the fully automated miniplot installation.

Publications:

B. Osmond, W. S. Chow, R. Wyber, A. Zavafer, B. Keller, B. J. Pogson, et al., "Relative functional and optical absorption cross-sections of PSII and other photosynthetic parameters monitored <i>in situ</i>, at a distance with a time resolution of a few seconds, using a prototype light induced fluorescence transient (LIFT) device," Functional Plant Biology, vol. 44, pp. 985-1006, 2017.

R. Pieruschka, H. Albrecht, O. Muller, J. A. Berry, D. Klimov, Z. S. Kolber, et al., "Daily and seasonal dynamics of remotely sensed photosynthetic efficiency in tree canopies," Tree Physiology, vol. 34, pp. 674-685, 2014.

A. Raesch, O. Muller, R. Pieruschka, and U. Rascher, "Field Observations with Laser-Induced Fluorescence Transient (LIFT) Method in Barley and Sugar Beet," Agriculture, vol. 4, p. 159, 2014.

G. Ananyev, Z. S. Kolber, D. Klimov, P. G. Falkowski, J. A. Berry, U. Rascher, et al., "Remote sensing of heterogeneity in photosynthetic efficiency, electron transport and dissipation of excess light in Populus deltoides stands under ambient and elevated CO2 concentrations, and in a tropical forest canopy, using a new laser-induced fluorescence transient device," Global Change Biology, vol. 11, pp. 1195-1206, 2005.

R. Pieruschka, D. Klimov, Z. S. Kolber, and J. A. Berry, "Monitoring of cold and light stress impact on photosynthesis by using the laser induced fluorescence transient (LIFT) approach," Functional Plant Biology, vol. 37, pp. 395-402, 2010.

Z. Kolber, D. Klimov, G. Ananyev, U. Rascher, J. Berry, and B. Osmond, "Measuring photosynthetic parameters at a distance: laser induced fluorescence transient (LIFT) method for remote measurements of photosynthesis in terrestrial vegetation," Photosynthesis Research, vol. 84, pp. 121-129, June 01 2005.



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