Dr. Agnes Csiszar

Senior Scientist

Forschungsthemen

Lipid-based nanoparticles, Molecular drug delivery, Cell membrane engineering, Model biomembranes

Kontakt

+49 2461/61-1415

+49 2461/61-3907

E-Mail

ORCID 0000-0002-8901-6804

Adresse

Forschungszentrum Jülich GmbH
Wilhelm-Johnen Str.
52428 Jülich
Germany

Institut für Biologische Informationsprozesse (IBI)

Mechanobiologie (IBI-2)

Gebäude 02.4 / Raum R 336

New strategy for breaking the cellular membrane barriers

Delivery of biomolecules to mammalian cells is a crucial step in all pharmacological treatments and most life sciences applications. Lipid nanoparticles or liposomes are frequently used for this purpose based on their high bioavailability and low toxicity. The main drawback of such liposomes is that their cellular uptake shows a strong preference for endocytosis. A faster and more effective alternative for efficient molecular delivery is the induction of membrane fusion between liposomes and mammalian cells, whereby the cellular plasma membrane merges with the liposomal membrane and the cellular cytoplasm with the liposomal lumen. Therefore, liposomes are highly appropriate for simultaneous and multiple live cell functionalization. Moreover, molecular delivery by fusion completely bypasses endosomes, avoiding cargo degradation. For this reason, our main goal has been to develop lipid-based nanoparticles with high membrane fusion ability and to use those particles as universal carriers for biomolecules in pharmacological and biomedical applications.

Vita

EMPLOYMENT HISTORY

04/2008 – Present

Senior Scientist | Forschungszentrum Jülich GmbH, IBI-2: Mechanobiology, Jülich, Germany

· Leader of the “Multifunctional Lipid-Nanoparticles” group

· Development of lipid-based drug delivery nanoparticles

· Development of biological model membranes

· Supervision of graduate and undergraduate students

· Involved in the teaching undergraduates

04/2004 – 03/208

Postdoctoral Fellow | Forschungszentrum Jülich GmbH, ICS-7: Biomechanics, Jülich, Germany

· Investigation of the mechanical properties of model biomembranes

· Development of a new model system for gram-negative bacteria wall

· Analysis of lipid membranes under osmotic stress

EDUCATION

09/2000 – 11/2003

RWTH Aachen | Aachen, Germany

PhD: Natural sciences

Supervisors: Professor Erwin Klumpp/Prof. Andreas Schaeffer

Thesis Title: Effect of 1,4-dichlorophenol on biological model membranes

09/1997 – 03/2001

Technical University Budapest | Budapest, Hungary

MSc: Teaching in Chemistry

Thesis Title: Didactics of spectroscopic methods in adult education

09/1997 – 08/1999

Technical University Budapest | Budapest, Hungary

MSc: Analytical Chemistry

Thesis Title: Effect of pesticide residues on lipid model membranes

09/1994 – 08/1997

Technical University Budapest | Budapest, Hungary

BSc: Chemical Engineering

Thesis Title: Analysis of textile fibers using IR-spectroscopy

Publikationen

1. Schmitt, Dreißen, Kolasinac, Csiszár, and Merkel (2022) Membrane tension controls the phase equilibrium in fusogenic liposomes, RSC Advances 12: 24114-24129

2. Kolasinac, Bier, Schmitt, Yabluchanskiy, Neumeier, Merkel, and Csiszár (2021) Delivery of the radionuclide 131I using cationic fusogenic liposomes as nanocarriers. Int J Mol Sci. 22 (1): 457

3. Wiedenhoeft, Braun, Springer, Teske, Noetzel, Merkel, and Csiszár (2020) The Basement Membrane in a 3D Breast Acini Model Modulates Delivery and Anti-Proliferative Effects of Liposomal Anthracyclines. Pharmaceuticals
13(9), 256

4. Kleusch, Monzel, Sridhar, Hoffmann, Csiszár, and Merkel (2020) Fluorescence correlation spectroscopy reveals interaction of some microdomain-associated lipids with cellular focal adhesion sites. Int J Mol Sci. 21 (21) 8149

5. Hoffmann, Hersch, Gerlach, Dreissen, Springer, Merkel, Csiszar, Hoffmann (2020) Complex Size and Surface Charge Determine Nucleic Acid Transfer by Fusogenic Liposomes, Int J Mol Sci. 21(6): 2244

6. Wiedenhoeft, Tarantini, Nyúl-Tóth, Yabluchanskiy, Csipo, Balasubramanian, Lipecz, Kiss, Csiszár, Csiszar, Ungvari (2019) Fusogenic liposomes effectively deliver resveratrol to the cerebral microcirculation and improve endothelium-dependent neurovascular coupling responses in aged mice. Geroscience, 41(6): 711

7. Hoffmann, Hersch, Merkel, Csiszar, Hoffmann (2019) Changing the way of entrance: highly efficient transfer of mRNA and siRNA via fusogenic nano-carriers, J of Biomed Nanotech. 15: 1-14

8. Kolasinac, Jaksch, Dreissen, Braeutigam, Merkel, and Csiszár (2019) Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells, Nanomaterials, 9 (7): 1025

9. Kolasinac, Kleusch, Braun, Merkel, and Csiszár (2018) Deciphering the Functional Composition of Fusogenic Liposomes, Int J Mol Sci. 19: 346

10. Kube, Hersch, Naumovska, Gensch, Hendriks, Franzen, Landvogt, Siebrasse, Kubitscheck, Hoffmann, Merkel, Csiszár (2017) Fusogenic Liposomes as Nanocarriers for Delivery of Intracellular Proteins, Langmuir 33: 1051

11. Braun, Kleusch, Naumovska, Merkel, and Csiszár (2016) A Bioanalytical Assay to Distinguish Cellular Uptake Routes for Liposomes, Cytometry A 89A: 301-308

12. Hersch, Wolters, Ungvari, Gautam, Deshpande, Merkel, Csiszar, Hoffmann, Csiszár (2016) Biotin-conjugated fusogenic liposomes for high-quality cell purification, J Biomater Appl. 6: 846

13. Csiszár, Csiszar, Pinto, Gautam, Kleusch, Hoffmann, Tucsek, Toth, Sonntag, Ungvari (2015) Resveratrol encapsulated in novel fusogenic liposomes activates Nrf2 and attenuates oxidative stress in cerebromicrovascular endothelial cells from aged rats, J Gerontol A 70:303

14. Naumovska, Ludwanowski, Hersch, Braun, Merkel, Hoffmann, Csiszár (2014) Plasma membrane functionalization using highly fusogenic immune activator liposomes, Acta Biomater. 10: 1403

15. Kleusch, Hersch, Hoffmann, Merkel, Csiszár (2012) Fluorescent lipids: functional parts of fusogenic liposomes and tools for cell membrane labeling and visualization, Molecules. 17: 1055

16. Csiszár, Hersch, Dieluweit, Biehl, Merkel, and Hoffmann (2010) Novel Fusogenic Liposomes for Fluorescent Cell Labeling and Membrane Modification, Bioconjugate Chemistry21: 537-543

Patents and patent pending:

1. Hoffmann, Csiszár, Hersch, Merkel: Mischung amphipathischer Moleküle und Verfahren zur Zellmembranmodifikation durch Fusion, 2015

Nr.: JP 2016504486

2. Hoffmann, Csiszár, Hersch, Merkel, Hoffmann, and Zantl: Method for producing a fusion mixture for transferring a charged molecule into and/or through a lipid membrane

Nr.: EP 3115039 A1

3. Hoffmann, Csiszár, Hersch, Merkel, Hoffmann, and Zantl: Method for producing a fusion mixture for transferring a charged molecule into and/or through a lipid membran

Nr.: US 20170009255 A1

4. Csiszár, Hoffmann, Braun: Verfahren zum Einbau von lysosomalen- Transmembranproteinen in zelluläre Membranen, 2015

Nr.: DE 102013005154 A1

5. Csiszár, Kleusch, Hoffmann, Merkel: Molecule mixture comprising an amphipathic molecule type a, which has a positive total charge in the hydrophilic range, and an amphipathic molecule type b and a polyphenol c, method for producing said molecule mixture and use thereof, 2017

Nr.: US 20150037398 A1

Letzte Änderung: 12.10.2023