Analytical Ultracentrifugation

The technique of analytical ultracentrifugation (AUC) was developed by Svedberg and Lysholm in 1927 (1). With AUC one is able to determine molecular weight, shape and stoichiometry of macromolecules and macromolecular complexes

Fluorescence correlation spectroscopy (FCS)

FCS is a method to detect molecular parameters like e.g. diffusion time, diffusion coefficent. Threrefore FCS can be used to analyse interaction of molecules (Elson & Magde, 1974; Magde et al., 1974; overview: Schwille & Haustein, 2001). In Fluorescence Correlation Spectroscopy the fluctuation of the fluorescence intensity is recorded in a very small volume, i.e. in femtoliter range via a confocal optic system.

NMR Spectroscopy

xtraordinary advances occurred during the last few years in structural biology by nuclear magnetic resonance (NMR) spectroscopy. The precision and accuracy of solution structures by NMR improved dramatically and the upper range of molecular weights accessible to NMR structure determination is still increasing.

Surface Plasmon Resonance (SPR)

This optical method allows detection and characterization of the binding of biological molecules

Phage display technologies

hage display technologies allow the identification of peptide ligands for a given target molecule out of a huge library of different peptides expressed on the surface of bacteriophages

Expression und Reinigung rekombinanter Proteine

Various biophysical methods require large amounts of recombinantly expressed and purified proteins

Atomic Force Microscopy

Atomic force microscopy is a technique used to mechanically scan surfaces at the nanometer to micrometer scale and measure atomic and molecular forces.

X-ray crystallography

The most widely used structural method for the determination of high-resolution structures of biological samples is X-ray crystallography.

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