Articles 2010 - 2018

The following manuscripts were published as a Group Leader at EMBL Heidelberg.


  • 44. Sachse C., Fromm S.A. Journal of Structural Biology – Paper of the Year 2018 J (2018) Struct Biol 204:369-370.
  • 43. Pellegrini, E. Desfosses, A., Wallmann, A., Schulze, W. M., Rehbein, K., Mas, L. Signor, P., Gaudon, S., Zenkeviciute, G., Hons, M., Malet, H., Gutsche, I., Sachse, C., Schoehn, G., Oschkinat, H., and Cusack, S. RIP2 filament formation is required for NOD2 dependent NF-κB signalling. (2018) Nat Commun, 9, (1) 4043.
  • 42. Zaffagnini, G., Savova., A., Danieli, A., Romanov, J., Tremel, S., Ebner, M., Peterbauer, T., Sztacho, M., Trapannone, R., Tarafder, A. K., Sachse, C. and Martens, S. Phasing out the bad-How SQSTM1/p62 sequesters ubiquitinated proteins for degradation by autophagy. (2018) Autophagy, 14 (7) 1280–1282.
  • 41. Zaffagnini, G., Savova., A., Danieli, A., Romanov, J., Tremel, S., Ebner, M., Peterbauer, T., Sztacho, M., Trapannone, R., Tarafder, A. K., Sachse, C. and Martens, S. p62 filaments capture and present ubiquitinated cargos for autophagy. (2018) EMBO J, p. e98308.
  • 40. Huber, S. T., Kuhm, T., Sachse, C. Automated tracing of helical assemblies from electron cryo-micrographs. (2018) J Struct Biol, 202 (1) 1–12.


  • 39. Jakobi, A. J., Wilmanns, M., and Sachse, C. (2017). Model-based local density sharpening of cryo-EM maps. eLife; 6:e27131.
  • 38. Sadian, Y., Tafur, L., Kosinski, J., Jakobi, A. J., Wetzel, R., Buczak, K., Hagen, W. J., Beck, M., Sachse, C., and Müller, C. W., Structural insights into transcription initiation by yeast RNA polymerase I. EMBO J, 36, (18) 2698–2709.
  • 37. Kolovou, A. , Schorb, M., Tarafder, A., Sachse, C., Schwab, Y., Santarella-Mellwig, R. (2017) A new method for cryo-sectioning cell monolayers using a correlative workflow. Methods Cell Biol., 140, 85–103.
  • 36. Dauden, M. I., Kosinski, J., Kolaj-Robin, O., Desfosses, A., Ori, A., Faux, C., Hoffmann, N. A., Onuma, O. F., Breunig, K. D., Beck, M., Sachse, C., Seraphin, B., Glatt, S. & Müller, C. W. (2017). Architecture of the yeast Elongator complex. EMBO Rep,18(2) 264–279.


  • 35. Tafur, L., Sadian, Y., Hoffmann, N. A., Jakobi, A. J., Wetzel, R., Hagen, W. J. H., Sachse, C., & Müller, C. W. (2016). Molecular Structures of Transcribing RNA Polymerase I. Mol Cell, 64(6) 1135–1143.
  • 34. Ohashi Y., Soler N., Garcia Ortegon M., Zhang L., Kirsten M. L., Perisic O., Masson G. R., Burke J. E., Jakobi A.J., Apostolakis A. A., Johnson C. M., Ohashi M., Ktistakis N. T., Sachse C., Williams R. L. (2016). Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34 complex. Autophagy, 12(11), 2129–2144.
  • 33. Schur, F.K.M., Obr, M., Hagen, W.J.H., Wan, W., Jakobi, A.J., Kirkpatrick, J.M., Sachse, C., Kräusslich, H.-G., Briggs J.A.G. (2016). An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation. (2016). An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation. Science, 353(6298), 506–508.
  • 32. Fromm, S.A., Sachse, C. (2016). Cryo-EM structure determination using segmented helical image reconstruction. Methods in Enzymology, 579, 307–328.
  • 31. Bertipaglia, C., Schneider, S., Tarafder, A. K., Jakobi, A. J., Bykov, Y. S., Picco, A., Kukulski W., Konsinski J., Hagen W.J.H., Wilmanns M., Briggs, J.A.G., Sachse, C. (2016). Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle. EMBO Rep, 17(7), 1044–1060.
  • 30. Hoffmann, N. A., Jakobi, A. J., Vorländer, M. K., Sachse, C., & Müller, C. W. (2016). Transcribing RNA polymerase III observed by electron cryo-microscopy. FEBS J, 283(15), 2811–2819.
  • 29. Klionsky, D. J., Abdelmohsen, K., Abe, A., Abedin, M. J., … Sachse, C. … et al. (2016). Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy, 12(1), 1–222.


  • 28. Hoffmann, N. A., Jakobi, A. J., Moreno-Morcillo, M., Glatt, S., Kosinski, J., Hagen, W. J. H., Sachse, C., Müller C. W. (2015). Molecular structures of unbound and transcribing RNA polymerase III. Nature, 528(7581), 231–236.
  • 27. Sachse, C. (2015). Single-particle based helical reconstruction—how to make the most of real and Fourier space. AIMS Biophysics, 2(2), 219–244.
  • 26. Johansen, T., & Sachse, C. (2015). The higher-order molecular organization of p62/SQSTM1. Oncotarget, 6(19), 16796-16797.
  • 25. Bharat, T.A.M., Murshudov, G.N., Sachse, C., and Löwe, J. (2015). Structures of actin-like ParM filaments show architecture of plasmid-segregating spindles. Nature, 523(7558), 106–110.
  • 24. Ciuffa R., Lamark T., Tarafder A., Guesdon A., Rybina S., Hagen W.J.H., Johansen T., Sachse C. (2015). The selective autophagy receptor p62 forms a flexible filamentous helical scaffold. Cell reports, 11(5), 748–758.
  • 23. Skruzny M., Desfosses A., Prinz S., Dodonova S.O., Gieras A., Uetrecht C., Jakobi A.J., Abella M., Hagen W.J.H., Schulz J., Meijers R., Rybin V., Briggs J.A.G., Sachse C., Kaksonen M., (2015). An organized co-assembly of clathrin adaptors is essential for endocytosis. Developmental cell, 33(2), 150–162.
  • 22. Gutsche, I., Desfosses, A., Effantin, G., Ling, W.L., Haupt, M., Ruigrok, R.W.H., Sachse, C., and Schoehn, G. (2015). Near-atomic cryo-EM structure of the helical measles virus nucleocapsid. Science, 348(6235), 704–707.
  • 21. Ehebauer, M.T., Zimmermann, M., Jakobi, A.J., Noens, E.E., Laubitz, D., Cichocki, B., Marrakchi, H., Lanéelle, M.-A., Daffé, M., Sachse, C., et al. (2015). Characterization of the Mycobacterial Acyl-CoA Carboxylase Holo Complexes Reveals Their Functional Expansion into Amino Acid Catabolism. PLoS Pathog 11, e1004623.
  • 20. Fromm, S.A., Bharat, T.A.M., Jakobi, A.J., Hagen, W.J.H., and Sachse, C. (2015). Seeing tobacco mosaic virus through direct electron detectors. J Struct Biol 189, 87–97.
  • 19. Elad, N., De Strooper, B., Lismont, S., Hagen, W., Veugelen, S., Arimon, M., Horre, K., Berezovska, O., Sachse, C., and Chavez-Gutierrez, L. (2015). The dynamic conformational landscape of  γ-secretase. J Cell Sci 128, 589–598.


  • 18. Vahokoski, J., Bhargav, S.P., Desfosses, A., Andreadaki, M., Kumpula, E.-P., Martinez, S.M., Ignatev, A., Lepper, S., Frischknecht, F., Sidén-Kiamos, I., Sachse, C. and Kursula, I. (2014). Structural differences explain diverse functions of Plasmodium actins. PLoS Pathog 10, e1004091.
  • 17. Afonin, S., Glaser, R.W., Sachse, C., Salgado, J., Wadhwani, P., and Ulrich, A.S. (2014). (19)F NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids. Biochim Biophys Acta 1838, 2260–2268.
  • 16. Desfosses, A., Ciuffa, R., Gutsche, I., and Sachse, C. (2014). SPRING – an image processing package for single-particle based helical reconstruction from electron cryomicrographs. J Struct Biol 185, 15–26.


  • 15. Gombos, L., Neuner, A., Berynskyy, M., Fava, L.L., Wade, R.C., Sachse, C., and Schiebel, E. (2013). GTP regulates the microtubule nucleation activity of γ-tubulin. Nat Cell Biol 15, 1317–1327.


  • 14. Bharat, T.A.M., Davey, N.E., Ulbrich, P., Riches, J.D., de Marco, A., Rumlova, M., Sachse, C., Ruml, T., and Briggs, J.A.G. (2012). Structure of the immature retroviral capsid at 8 Å resolution by cryo-electron microscopy. Nature 487, 385–389.
  • 13. Guichard, P., Desfosses, A., Maheshwari, A., Hachet, V., Dietrich, C., Brune, A., Ishikawa, T., Sachse, C., and Gönczy, P. (2012). Cartwheel architecture of Trichonympha basal body. Science 337, 553.


  • 12. Korkhov, V.M., Sachse, C., Short, J.M., and Tate, C.G. (2010). Three-dimensional structure of TspO by electron cryomicroscopy of helical crystals. Structure 18, 677–687.
  • 11. Sachse, C., Grigorieff, N., and Fändrich, M. (2010). Nanoscale flexibility parameters of Alzheimer amyloid fibrils determined by electron cryo-microscopy. Angew Chem Int Ed Engl 49, 1321–1323.
Last Modified: 01.05.2022