Liquid-Liquid Phase Separation in biological materials
Non-membrane bound organelles carry out cellular function and allow rapid response to the environment and stimulus. They form by liquid-liquid phase separation of highly flexible proteins (e.g. IDP), nucleic acids, additives and substrates. Due to structural flexibility and composition distinct micro environments and solvent properties allow process specific substrates combined with an easy access of the active region. The liquid-liquid phase transition of protein solutions is influenced by composition and salt concentration. Our goal is to elucidate the structure, dynamics and molecular transport of/in the liquid phase starting from polyaminoacids in different composition, over disordered protein polymers to IDP forming the liquid phase.