Standard Techniques in Biochemistry and Biophysics

F. Müller (ICS)
(building 15.1, room 249)

In most projects that involve biological macromolecules, various standard techniques, such as absorption, CD, or fluorescence spectroscopy, light scattering, calorimetry, chromatography, centrifugation, and computer simulations, are employed to characterize or even to prepare the samples. On the one hand, these standard techniques are often a basic prerequisite before more advanced and elaborated methods can be applied, such as x-ray crystallography, NMR, and cryo-EM. On the other hand, results that have been obtained using these standard techniques are already valuable by themselves as part of an approach that employs many methods simultaneously. In this lecture series, the following techniques will be presented:

• Absorption, CD, and basic fluorescence spectroscopy (Jörg Fitter)
  Wednesday, April 29th, 1:00pm-2:30pm (building 02.4w, room 309b)
• Bioinformatic tools: sequence analysis, homology modeling, display, and modeling of protein structures (Gunnar Schröder)
  Wednesday, May 5th, 1:00pm-2:30pm (building 05.2, room 3012)
• Advanced flourescence techniques: protein labeling, FCS, anisotropy (J. Fitter)
  Wednesday, June 17th, 1:00pm-2:30pm
• Differential scanning calorimetry (DSC) and isothermal titration calorimetry (Bernd König, Wolfgang Hoyer)
  Wednesday, July 1st, 1:00pm-2:30pm
• Special talk on a recent project in the institute for Cellular Biology:
  Anion permeation through excitatory amino acid transporters (Jan-Philipp Machtens)
  Wednesday, July 1st, 3:00pm-4:30pm
  Recent research in an IHRS BioSoft group related to the lecture series that has been highlighted in a press release (in German language) and that is discussed on f1000.com. This talk is not part of the lecture series.
• Surface plasmon resonance (Biacore), thermophoresis (Kerstin Reiß)
  Wednesday, July 22nd, 1:00pm-2:30pm