Methodology and Lab Equipment

Students in our laboratory can expect to gain experience in several interesting and modern techniques and methods, including:

• Molecular microbiology: Cloning techniques, site-directed mutagenesis, Gibson assembly

• Phage biology: Isolation, sequencing, assembly and annotation, infection studies

• Single-cell analysis: time-lapse fluorescence microscopy (Live Cell Imaging), flow cytometry and cell sorting (FACS)

• OMICS techniques: in particular genomics, transcriptomics (microarrays & RNA-Seq), ChIP-Seq, ChAP-Seq (Fig. 5), and proteomics

• Next-generation sequencing: genome re-sequencing and RNA-Seq (Illumina MiSeq, Nanopore)

• Bioinformatics & data science: analysis and statistical verification of scientific datasets using Python, R and in-house developed pipelines