Trimmomatic | Trimm and Preprocess NGS Data

Trimmomatic is a command line tool that performs a variety of functions for Illumina paired-end and single-end sequencing data, including sequence trimming and adapter removal. It is a flexible and efficient NGS preprocessing tool, which greatly improves downstream applications.

Download: Trimmomatic

Contact: Marie Bolger

More Information:

Trimmomatic: a decade of feature-rich, high-performance NGS read preprocessing Sebastian Beier,  Anthony M. Bolger,  Marie E. Bolger,  Rainer Schwacke,  Björn Usadel. Bioinformatics, Volume 42, Issue 6, June 2026, btag331, https://doi.org/10.1093/bioinformatics/btag331

Bolger, A. M., Lohse, M., & Usadel, B. (2014). Trimmomatic: A flexible trimmer for Illumina Sequence Data. Bioinformatics, btu170.