Methods and Technologies at the
Jülich Plant Phenotyping Centre
GROW MAP-leaf
Digital Image Sequence Processing Setup for monitoring of leaf growth via digital image sequence processing
Spatio-temporal quantification of leaf area growth
Ajustment of lights and camera
Data Acquisition
- fixation of a leaf in a plane parallel to camera
- illumination with near infrared-diodes
- image sequence at high frequency in near infrared throughout day and night
Set up and evaluation of an experiment with GROW Map-Leaf
Image analysis
- optical flow analysis of image sequence resulting in velocity vector fields
- calculation of divergence to obtain growth maps
Results example
Spatial and temporal distribution of leaf growth in 4 species ilustrating typical growth patterns
Literature
Wiese, A.; Christ, M. M.; Virnich, O.; Schurr, U. and Walter, A. (2007) Spatio-temporal leaf growth patterns of Arabidopsis thaliana and evidence for sugar control of the diel leaf growth cycle.
New Phytologist 174, 752-761