Methods and Technologies at the
Jülich Plant Phenotyping Centre

GROW MAP-leaf

Ajustment of lights and camera

Data Acquisition

• fixation of a leaf in a plane parallel to camera
• illumination with near infrared-diodes
• image sequence at high frequency in near infrared throughout day and night

Set up and evaluation of an experiment with GROW Map-Leaf

Image analysis

• optical flow analysis of image sequence resulting in velocity vector fields
• calculation of divergence to obtain growth maps

Results example

Spatial and temporal distribution of leaf growth in 4 species ilustrating typical growth patterns

Literature

Walter A and Schurr U (2005) Dynamics of leaf and root growth: endogenous control versus environmental Impact. Annals of Botany 95: 891–900

Wiese, A.; Christ, M. M.; Virnich, O.; Schurr, U. and Walter, A. (2007) Spatio-temporal leaf growth patterns of Arabidopsis thaliana and evidence for sugar control of the diel  leaf growth cycle.
New Phytologist 174, 752-761