Malonyl-CoA in and secondary metabolites from coryneform bacteria

TO-151 • PT 1.2829• As of 2/2024
Institute of Bio- and Geosciences
Biotechnology (IBG-1)

System for providing malonyl-CoA in coryneform bacteria and process for producing e.g. polyphenols and polyketides with coryneform bacteria

Technology

The invention comprises a coryneform bacterial cell with an increased intracellular supply of malonyl-CoA compared to the original wild-type cell as well as a protein for a fatty acid FasB with reduced functionality and the nucleic acid sequence coding for it.

Problem addressed

The chemical group of (poly)phenols and polyketides includes a wide variety of molecules that have great potential for pharmacological or biotechnological applications. However, these substances are only produced in very small quantities by their natural producers or the producers cannot be cultivated. One described microbial route for production uses the bacterium Escherichia coli, which is particularly questionable for use in medicine.

Solution

With the present system and process, the secondary metabolites can be produced microbially and on an industrial scale in microorganisms that are recognized as safe. This is made possible by the increased supply of malonyl-CoA, a crucial building block of polyphenols and polyketides.

Benefits and Potential Use

Microbial, large-scale production of polyphenols and polyketides.

Development Status and Next Steps

The functionality has been proven on a laboratory scale.

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