Alterations in the Brain Under the Neutron Magnifying Glass

Jülich / Garching, 23 February 2022 - The brain is the centre of our nervous system - structural changes are often involved in neurological diseases and mental disorders. A team from Forschungszentrum Jülich has now developed a neutron-based method at the Heinz Maier-Leibnitz Zentrum (MLZ) to study brain slices and gain a better understanding of these types of diseases.

The brain can be divided into so-called grey matter and white matter. The white matter contains the axons that transmit stimuli. For faster stimulus transmission, the axons are wrapped in an insulating layer of myelin, in the same way as a cable is wrapped in rubber insulation to ensure that no electricity is lost along the way.

3D-Polarized Light Imaging Aufnahme eines Maus-Gehirns. Die strukturelle Information über die Ausrichtung der Nervenfasern aus dieser Aufnahme dienten als Vergleich für die neuen Neutronen-Messungen.
3D-Polarized Light Imaging Aufnahme eines Maus-Gehirns. Die strukturelle Information über die Ausrichtung der Nervenfasern aus dieser Aufnahme dienten als Vergleich für die neuen Neutronen-Messungen.
Axer, Amunts et al. / INM-1, Forschungszentrum Jülich

If the myelin sheath is damaged or degraded, this leads to impaired brain and body functions. In multiple sclerosis, for example, the insulating myelin layer is severely impaired. However, the exact causes of the disease are still unclear.

Researchers at the Jülich Institute of Neuroscience and Medicine and the Jülich Centre for Neutron Science have developed a new imaging method at the small-angle scattering facility KWS-1 at the MLZ to map the density, structure, and spatial orientation of nerve fibres and myelin.

New method complements previous procedures

"For a long time now, it has been possible to determine the spatial orientation of nerve fibres in brain slices using light microscopy and polarization," explains Dr. Heinrich Frielinghaus from the JCNS, head of the KWS-1 group, "but the exact structure and orientation of the myelin on the molecular scale cannot be detected using light." With the help of neutrons, the researchers can now for the first time determine both structure and orientation, and correlate these with polarization measurements.

Schema des experimentellen Aufbaus an der Kleinwinkelstreuanlage. Der Neutronenstrahl trifft auf einen Gehirnschnitt („Sample“). Ein Detektor zeichnet die Streuung auf.
Schema des experimentellen Aufbaus an der Kleinwinkelstreuanlage. Der Neutronenstrahl trifft auf einen Gehirnschnitt („Sample“). Ein Detektor zeichnet die Streuung auf.
Forschungszentrum Jülich

The special feature of this new method is that it allows scientists to image the axons bundled into nerve fibers at the same time as the insulating myelin sheath.

"With our neutron-based method, we can confirm the results of previous methods and provide additional information," says Frielinghaus.

Applications in medicine

"Since we work with brain slices, it is of course not a diagnostic procedure," explains Frielinghaus. With their new method, the researchers hope to better understand the causes of neurological diseases in the future by being able to visualize structural changes in the brain more completely.

Dr. Heinrich Frielinghaus an der Kleinwinkelstreuanlage.
Dr. Heinrich Frielinghaus an der Kleinwinkelstreuanlage.
Andreas Heddergott/TUM

The resolution of the new method is still in the millimeter range. However, Frielinghaus and his colleagues expect that measurements precise to the micrometer will be possible in the future. This will enable them to study even the smallest structures in biological tissues.

(Veronika Aechter, FRMII / TUM)

Original publication
S. Maiti, H. Frielinghaus, D. Gräßel, M. Dulle, M. Axer, S. Förster, Distribution and orientation
of nerve fibers and myelin assembly in a brain section retrieved by small‐angle neutron scattering, Sci Rep 11 (2021) DOI: 10.1038/s41598-021-92995-2

Further Information

Jülich Centre for Neutron Science

Heinz Maier-Leibnitz Zentrum

Institute for Neuroscience and Medicine – INM-1, Fibre architecture

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Contact

Dr. Henrich Frielinghaus
Forschungszentrum Jülich
Jülich Centre for Neutron Science am Heinz Maier-Leibnitz Zentrum (JCNS-MLZ)
Tel.: +089 158860-706
E-Mail: h.frielinghaus@fz-juelich.de

Last Modified: 29.10.2022